DK79 Posted October 30, 2017 Posted October 30, 2017 I have performed a binding experiment using a biotynilated molecule ranging from high to low concentration. The molecule exhibits 100% binding at low concentration (as expected) then gradually drops off. The drop -off is more extreme at higher concentrations. If the beads become saturated past a certain concentration shouldnt the curve drop off quicker and at a steady rate? (it should be noted that the % captured is measured by fluorescence) Binding curve is below. The beads are GE's Sepharose Mag beads cat # 28-9857-38 Thanks!
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