TinyBby69 Posted December 14, 2017 Posted December 14, 2017 its catalase +, oxidase +, ferments glucose and produces gas from the process, and is a gram + facultative anaerobe and its highly resistant to antibiotics and motile + and has been seen to grow up to temps of 103°F, colonies are white and turn slightly yellowish, orange after 2-3 days, small punctiform colonies. Colony elevation is raised. Colony margin varies from complete to irregular, odor is pungent and has an odor of ammonia after several days
Paul Atreides Posted December 16, 2017 Posted December 16, 2017 Are you sure that ammonia is produced from these bacterial colonies? From my reading, it seems that bacteria do not produce ammonia rather they consume it and produce nitrates that give a sharp-acidic smell (pungent, in other words) that may be confused as ammonia. The class of bacteria you have examined are therefore nitrifying bacteria, but I am having trouble trying to identify which sub-class of bacteria you are trying to view. It would be more helpful if you could provide information on how you obtained these colonies and what growth medium you used (also, did you use any colorimetric methods?), as it is common knowledge that the growth medium can have an effect on the colour of the colonies e.g. mannitol salts agar when fermented give yellow colonies. I had found a few possible suspects, but they are lacking in certain ways based on the descriptions you had given. The colonies best fit the descriptions of the genus Nitrosomonas, but this genus is mostly composed of gram-negative not gram-positive bacteria.
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