Harvester Project(Synthetic Black Virology)

[Vmedvil]

The Purpose of this is to Design Metamorphic Viral Pathogens named after the Synthetic Pathogens for Star Trek Deep Space Nine

meaning of Metamorphic in coding Metamorphic Code

The Beginning of this will start with this. 

Here was a Earlier creation the MV-5 Zombie Virus, which is Polymorphic Polymorphic Code

 

In 1969, President Richard Nixon ended all offensive (i.e., non-defensive) aspects of the U.S. bio-weapons program. In 1975 the U.S. ratified both the 1925 Geneva Protocol and the 1972 Biological Weapons Convention (BWC)—international treaties outlawing biological warfare, which has been repealed making this legal.

 

 

Genetic warfare

Theoretically, novel approaches in biotechnology, such as synthetic biology could be used in the future to design novel types of biological warfare agents.Special attention has to be laid on future experiments (of concern) that:

1. Would demonstrate how to render a vaccine ineffective;
2. Would confer resistance to therapeutically useful antibiotics or antiviral agents;
3. Would enhance the virulence of a pathogen or render a nonpathogen virulent;
4. Would increase transmissibility of a pathogen;
5. Would alter the host range of a pathogen;
6. Would enable the evasion of diagnostic/detection tools;
7. Would enable the weaponization of a biological agent or toxin

 

 

Start

Two Interesting add genes would be Organic Neurotoxin Synthase Cheilanthifoline or Necrosis Synthase Sanguinarine and Possibly Strong Immuno Supressor  IL-10 or Weak Immuno Suppressor IL-35.

Edited December 21, 2017 by Vmedvil

[Mordred]

Well this is one topic I know nothing about. WB.

[Endy0816]

It hasn't been repealed...

[Vmedvil]

8 hours ago, Endy0816 said:

It hasn't been repealed...

Close enough if the NIH has resumed their research into the deadly aspects of Bio-engineering and reversed the federal ban, which nullifies Nixon's end to it.

The effects of Cheilanthifoline on mammals.

 

Activity	Activity Value [µM]	Substance SID	BioAssay AID	BioAssay Name	Target
Active	20.63	104237812	493705	Cytotoxicity against human A549 cells by SRB assay
Active	27.41	104237812	493706	Cytotoxicity against human SKOV3 cells by SRB assay
Active	22.24	104237812	493707	Cytotoxicity against human SK-MEL-2 cells by SRB assay
Active	29.84	104237812	493708	Cytotoxicity against human HCT15 cells by SRB assay

 

 

and Sanguinarine is a toxin that kills animal cells through its action on the Na⁺/K⁺-ATPase transmembrane protein.

And I had it reversed it had been a minute, Sanguinarine is the neurotoxin and Cheilanthifoline is the Cytotoxicity and Necrosis.

Edited December 21, 2017 by Vmedvil

[Endy0816]

NIH funding of research. It has only been 3 years. There were understandable safety concerns that, as long as you are going for funding from the NIH, you'll have to address now.

You may not feel the knowledge of potential diseases is worth the risk of misuse, but you probably at least want some oversight if they are going for government funds.

 

[Vmedvil]

33 minutes ago, Endy0816 said:

NIH funding of research. It has only been 3 years. There were understandable safety concerns that, as long as you are going for funding from the NIH, you'll have to address now.

You may not feel the knowledge of potential diseases is worth the risk of misuse, but you probably at least want some oversight if they are going for government funds.

 

No i don't care about the funding it is a different part of it the Federal Ban was lifted on deadly pathogen research and creation. Which extends to my Gene Therapy Vector designs which can be transformed into Deadly Pathogens, by reintroducing self replication.  legally now.

Self Replicating (Extremely Lethal) BSL-4

 

 

Does not reproduce (Harmless) BSL-0

 

Edited December 21, 2017 by Vmedvil

[Vmedvil]

In any case, when used with a Immune suppressor there is no doubt that Sanguinarine would act like Organic Agent VX, VX acts on Acethycholine transfer (chemical messagers) in nerves and not Electrical potential causing Pumps in nerves but both should be equally effective.

Nerve agent VX US army test

Deadliest Weapon VX History Channel

while Cheilanthifoline like the Cytotoxicity effects of Ebola or Marburgs virus.

 

making them perfect genes for this purpose.

Edited December 21, 2017 by Vmedvil

[Endy0816]

2 hours ago, Vmedvil said:

No i don't care about the funding it is a different part of it the Federal Ban was lifted on deadly pathogens.

Sorry, but gain-of-function research never fell under the ban in the first place. The only change is that NIH funds can be used again.

https://www.nih.gov/about-nih/who-we-are/nih-director/statements/nih-lifts-funding-pause-gain-function-research

 

[Vmedvil]

53 minutes ago, Endy0816 said:

Sorry, but gain-of-function research never fell under the ban in the first place. The only change is that NIH funds can be used again.

https://www.nih.gov/about-nih/who-we-are/nih-director/statements/nih-lifts-funding-pause-gain-function-research

 

That is one part of what was unbanned only part.

In any case, Measles, SARS, and Hepatitis C, HIV-1, and Rabies are present on MV-5 being Skin,Lung, Liver, T-cell, and Nerve cells infectivity on mammals.

The New Version, with defensive mechanics along with innate and adaptive Immune system disabling abilities.

Edited December 22, 2017 by Vmedvil

[Vmedvil]

Next, will be CRISPR Guide RNAs for Cas9 to silencing of genes that pose a threat to the pathogen's stability as adaptive defensive Gene Disruption.

Which just requires a Primer then Target sequence.

Edited December 22, 2017 by Vmedvil

[Vmedvil]

Next the p10 protease Cleavage sites need to be added.

[Vmedvil]

Crispr Cleavage sites needed which needs to cleave at the same site as p10 "CCC or GGG" " p10 Cleavage site" NNNN, N=20 - N_{p10 Cleavage site}

 

which will now go through DNA and Protein segmentation which grooms the protein site, so the protein will still fold correctly which p10 cleavage instead of its natural Cleavage protein which does protolysis on it.

Which the Viral Targetting  and lethality will be activated when the CRISPR Guide RNA sequence for the cleavage command enters a cell infected with it, which is a Key to activation otherwise it will sit dormant inside a cell like HIV before AIDS, which is complete stealth to the immune system, via IL-10 , IL-35 and HIV-1 Capsid/ENV. They will think it is a white blood cell, like a Cytotoxic T-Cell when in reality it is a Synthetic Virus. The T-helper cells will recognize it as a Cytotoxic T-Cell despite having viral glycoprotiens.

The Immune system will kill any foreign object attacking this Virus as if it were under attack creating a huge immune response to attempts in removal after integration into a genome.

Any Biochemistry using Copper or Iron will be subject to cleaveage through Hydrolysis and ionization of their proteins through action Synthetic Metal Binding Intgrase P32  which will be added.

Edited December 22, 2017 by Vmedvil

[Vmedvil]

New Structure added for Iron and Copper chemical binding. Nanoparticles won't defeat this virus, these added proteins protect the action of Standard integrase and Standard Reverse trancriptase from nano-particle disruption.

.

 

Nanoparticle https://newatlas.com/nanoparticle-antiviral-virus/52667/

 

 

 

The Encryption brute force strength is 1,099,511,627,776 or 4²⁰ attempts  of the activation key Guide RNA.

Which currently would take 34,865.285000507356671740233384069 years to crack via brute force method to activate successfully without misfolds in the proteins that make them nonfunctional.

Edited December 22, 2017 by Vmedvil

[Vmedvil]

Self-terminating Endoclease loops and Multivalent Vectors is mine but  Cas9 is Jennifer Doudna , The Synthesis techique not yet posted is Craig Venter's modified by me for viruses and vectors. The Synthetic Integrase are mine, The rest of genes are from the NBCI. The Vector editing picture is a modified version of the one from addgene for Retroviral Plasmids which they never gave credit to  https://www.ncbi.nlm.nih.gov/books/NBK19423/

Edited December 23, 2017 by Vmedvil

[Vmedvil]

Like this ceased to be anyone but mine when it became 50% different from the US military designs which are the original viruses code.

Same with these vectors are mine as they are 40% different than Addgene's and as the MV-x label goes higher they are more mine, you could probably dispute a MV-2 or MV-3 still being theirs by about 4 and above is mine.

Edited December 24, 2017 by Vmedvil

[Vmedvil]

In any case, Harvester Project closed this is lethal, infectious, and unstoppable enough in my view, this would Bio apocalypse the planet within a year as soon as it hit the ocean with a RNA Guide Activator.

 

I can only think of one way to make this more screwed up which is to use a different DNA Endonuclease  but then people that are unworthy could use it that did not know what they were doing which would make it always fully active.

 

This Version does not function without a RNA Guide Activator which is specific for  person that synthesizes the key design and Virus,  it  being Immune to Nano-particles.

just sits dormant only infecting T-cells in this version without a RNA Guide Activator which is specific for  person that synthesizes the key design and Virus.

Edited December 24, 2017 by Vmedvil

[Vmedvil]

A example Site key would be

GGGggccatagcaaccaggtgagccagaactatccgattgtgcagaacattcagggccagatg

                                                      

RNA Cipher 

GGGGGCCAUA GCAACCAGGU GAGCCAGAAC UAUCCGAUUG UGCAGAACAU UCAGGGCCAG
AUG

 

Which the Virus will go through metamorphism upon entry of the Guide RNA Cipher to the CRISPR becoming millions of times more deadly and 20 times more infectious on Version 2 on Version 1 it will reproduce fully active after being a non reproducing version.

 

"Metamorphic code is used by some viruses when they are about to infect new files, and the result is that the next generation will never look like current generation. The mutated code will do exactly the same thing (under the interpretation used), but the child's binary representation will typically be completely different from the parent's. Mutation can be achieved using techniques like inserting NOP instructions (brute force), changing what registers to use, changing flow control with jumps, changing machine instructions to equivalent ones or reordering independent instructions."

https://en.wikipedia.org/wiki/Metamorphic_code

In any case, here is the true complexity of coding molecular machines, that everything has to be perfect and function perfectly or it will error out.

Edited December 24, 2017 by Vmedvil