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Depends what "use" you are thinking of.

DNA can create proteins without being compared with anything.

We can work out what certain genes do by looking at the effects of different versions (alleles). Not sure if that is covered by your definition of "comparing".

To identify someone from DNA, we have to have their (or a close relative's) DNA to compare with. It might be possible, one day, to go directly from the DNA to a picture/profile of the person.

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