converting umol/min/umol to umol/min/mg

[northernlad2690]

Hello, I'm just wanting someone to give me a little bit of guidance on the following conversion,

i'm trying to convert my reading of umol/min/umol to umol/min/mg- i think i have the correct answer but i need my method validated if someone could possibly help?

i have a spectrophotometric assay of a purified recombinant enzyme, Spy057, against p-nitrophenyl N-acetyl-β-glucosaminide substrate (total reaction volume, 2 mL; volume of Spy05783 added, 50 µL):

- I have calculated the umol/min/umol using beer lamberts law to 289.63 

- the Mr of spy057 is 43507g/mol

- i then calculated that there is 43.507mg/umol

- there is 1.899 x 10E-4 umol added reaction volume 

so using this i then calculated 43.507x(1.899E-4) = 8.272 x 10E-3 

using data obtained previously i knew that the umol/min of p-nitrophenol was 0.055 so this was input into the final equation 0.055/8.272 x 10E-3 giving me 6.657umol/min/mg

does this look correct, ive done everything to try and find a protocol but cant find anything

 

 

[studiot]

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