Granulosa Cell Culture and Unidentified Sitting Objects in Flask

[dr.szirh]

Hi to all researchers.

We have 2 problems in our lab. First is there any researcher made granulosa cell culture? We could not culture cells than 15 days. Second we saw unidentified attached or swimming geometric objects. (in pictures) Can anyone identidy theese structures? Thanks for your helps...

[CharonY]

Can't give you tips on the cell culture, but the geometric objects look like salt crystals to me. I.e. something is precipitating out of solution, which might be a reason why your culture does not work.