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Functions of different promotors in PinPoint cloning plasmids


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Posted (edited)
Hello,
 
In this PinPoint Xa-1 Vector, there are 3 different promotors (T7-promotor, tac-promotor and SP6-promotor). I'm confused why the T7-promotor and the tac-promotor are placed immediately after each other. Also, why is the SP6-promotor placed in opposite direction at the end of the MCS?
 
Thanks!

pinpoint.png

Edited by Geox

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