Application of same antibody, in different concentrations

[SgtCheesyBread]

Hello,

I have a question which may be trivial but I cannot seem to wrap my head around it: I ordered a certain antibody from an US american vendor, which was delivered to me in form of a powder and I dilluted it to 1mg/1ml for my experiments. I used 50µl to achieve that concentration, just as suggested by the vendor. I used samples with 0.2µl, 2µl and 4µl of my AB solution for my experiments, and it worked perfectly fine.

MY antibody ran out and I wanted to ordered some more, problem was, the vendor changed their product and they are only selling the antibody in a concentration of 0.1mg/1ml anymore. I know the formula c1V1 = c2V2. Would that mean I have to use ten times the amount to achieve the same results in my experiments? Meaning, 2, 20 and 40µl?  Thank you very much for your help.

Greetings,

[CharonY]

It is easiest just to look at the concentration. In the original vial you have 1mg in 1 mL solution. In the second vial you got 0.1mg in the same volume, which is (as you correctly pointed out) a tenth of 1 mg. Using a formula seems to complicate things here in my mind.