GVE Posted September 18, 2019 Posted September 18, 2019 Aspartate aminotransferase (AAT) enzyme was being purified from pigs heart. Tissues were homogenated and cells were eliminated with gel filtration. Nucleic acids were precipitated with polyethylenimine and extract1 was received with volume 2dm3. Then 50microLitres of this extract1 were added to 3cm3 buffer and optical density was measured at 280nm (1cm light path) and the value of it was 1,7. How should I count the amount of protein in extract1?
BabcockHall Posted September 19, 2019 Posted September 19, 2019 What do you know about using Beer's law? I have a feeling that you will only be able to obtain an approximate answer, but it is better than nothing.
Sensei Posted September 19, 2019 Posted September 19, 2019 58 minutes ago, BabcockHall said: What do you know about using Beer's law? "do not overdo it with alcohol because you will have a hangover"..
BabcockHall Posted September 26, 2019 Posted September 26, 2019 To a rough approximation, when one has 1 mg/mL of protein, the absorbance at 280 nm will be 1 AU. Obviously this depends on the protein.
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