Pipetting "the last drop"

[nuck_chorris]

Howdy,

Recently I have been practicing pipetting small volumes (approx 10 uL). I have been holding the pipette at a 45 degree angle, and I don't normally have the problem with larger volumes.

I make sure to press all the way down but still somehow find a way to let the very last bit to come back into the tip upon release.

This is also in particular with a single volume pipette. My only thought is that maybe I am pressing the tip too much against the wall? 

Any help would be appreciated,

Nuck Chorris

[studiot]

Something like this  ?

[CharonY]

Try to pipette into existing solution if possible. Lacking that, press the press all the way down, then use wall of the tube to release the last drop. Pull the pipettor up and then slowly release the plunger.

[Santhoshkumar]

Simple by releasing the solution to get down just stop the flow before the last 1ml letting go and leave it now

So by stopping the flow and allowing again to flow leads to create over pressure there and so you may not get that difficulty