Which plasmid can express T7 RNA polymerase in mammalian cells?

[wenhu]

Hi,

Does anyone know what plasmid could express T7 RNA polymerase in mammalian cells, I would like to transfect it with other plasmid containing Gene Of Interest under control of T7 promoter. Thanks a lot!

[Dagl1]

I suppose you just transfect your cells with a T7 RNA polymerase expression plasmid (my apologies if it turns out that this doesn't work, I didn't look up any difficulties).
I found: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171830/ You may want to use some of their methods.

[wenhu]

14 hours ago, Dagl1 said:

I suppose you just transfect your cells with a T7 RNA polymerase expression plasmid (my apologies if it turns out that this doesn't work, I didn't look up any difficulties).
I found: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171830/ You may want to use some of their methods.

Thank you Dagl1, I have already noticed that. Since this is made by some lab, I wonder if there are some commercial off-the-shelf plasmid.

[Dagl1]

You can always check addgene: https://www.addgene.org/59926/ (I think this one is okay but only quickly looked, please investigate before buying :)!)
Thermofisher, sigma and origene also sell vectors or expression systems, however I couldn't easily find an eukaryotic expression vector there.