Question on primer design

[Molbio_learning]

Hello everyone, this is my first post and I am hoping it is the right place to ask.

I just recently started cloning, PCR design and similar. I have very basic question and would like to get an answer step by step if possible, using an example 🙏🏻
So I have a pool of oligos (with short stretch of the same sequence at 5’ and 3’ so I can do PCR with it, for example to introduce nuclease sites). My question is, how do I design a primer for PCR to introduce biotin site and let’s say one of any restriction enzymes?? 
 

I hope I provided enough of info, I need only one primer.

thanks a lot!!! 🤓

[CharonY]

If you want your product to be biotinylated, you need to attach a biotin. This can be done directly during synthesis (i.e. order it if you get them commercially), but there are also labelling kits if for some reasons you want to do it on your own. 

If you want to get a new restriction enzyme site, you need to add it to your oligo upstream of your priming site. I think "Molecular Cloning" (Green and Sambrook) should have chapter about it, but it is fairly common nowadays and I would think that many suppliers (e.g. NEB) will have information about that (though of course they also want to peddle their specific products, you can ignore that).

[Molbio_learning]

Thank you for the reply. Yes, I want to get biotinylated product directly during synthesis, how do I order such primer?