D antigen & biological immortality

[Bufofrog]

You probably didn't notice but this is an English language site.  Since your immortal couldn't you spare a little time to learn English?  Its not like your pressed for time, sheesh!

[Flamboyant]

https://www.collinsdictionary.com/dictionary/english/immortal

Word forms: plural immortals

1. adjective

Someone or something that is immortal is famous and likely to be remembered for a long time.

...the immortal Reverend Dr Spooner.

...Wuthering Heights, Emily Brontë's immortal love story.

Maybe my work is not immortal, but it will live for a while.
 

Synonyms: timeless, eternal, everlasting, lasting   More Synonyms of immortal

An immortal is someone who is immortal.

He called Moore 'one of the immortals of soccer'. [+ of]

immortality (ɪmɔːʳtælɪti ) uncountable noun

Some people want to achieve immortality through their works.
 

Synonyms: fame, glory, celebrity, greatness   More Synonyms of immortal

2. adjective

Someone or something that is immortal will live or last for ever and never die or be destroyed.

The pharaohs were considered gods and therefore immortal.

Synonyms: undying, eternal, perpetual, indestructible   More Synonyms of immortal

 

An immortal is an immortal being.
 

...porcelain figurines of the Chinese immortals.

immortality uncountable noun

The Greeks accepted belief in the immortality of the soul. [+ of]

Synonyms: eternity, perpetuity, everlasting life, timelessness   More Synonyms of immortal

3. adjective [ADJECTIVE noun]

If you refer to someone's immortal words, you mean that what they said is well-known, and you are usually about to quote it.

...Roosevelt's immortal words, 'Speak softly and carry a big stick.

Edited June 30, 2021 by Flamboyant

[Flamboyant]

What the... hell ? I thought D-antigen was the problem ; never thought about T-antigen. Please, can someone explain to me the difference ?

Immortality is the indefinite continuation of a person's existence, even after death. In common parlance, immortality is virtually indistinguishable from afterlife, but philosophically speaking, they are not identical.

Immortality is the ability to live forever, or eternal life. ... The absence of aging would provide humans with biological immortality, but not invulnerability to death by physical trauma.

Can an immortal person be killed ?

Paradoxical though it might seem, biologically immortal organisms are definitely mortal. They can be killed by a predator, a disease, or a catastrophic change in the environment such as an erupting volcano. But unlike humans, they rarely die simply because they get old.

Can someone explain to me why the words "rarely die" ? As if we had examples...

SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts : https://pubmed.ncbi.nlm.nih.gov/1851674/
 

Quote

Two lines of immortal human fibroblasts were isolated following transfection of TIG-3 cells with plasmid DNA, pMT-1ODtsA, that contained SV40 early gene with a deletion in replication origin and its mutation in coding sequence for T-antigen. These cells continued proliferation at 34 degrees C, over 565 population doubling level (PDL) which is far over the limited division potential of untransformed normal TIG-3 of 70-80 PDL. When the culture temperature was shifted to 40 degrees C after 70 PDL, they ceased proliferation immediately. One of these immortal clones, SVts8, lost its ts phenotype after retransformation with wtT-antigen gene. These results indicated that the function of intact T-antigen is required for maintenance of immortal proliferation, at least in one of the SV40 transformed immortal clones.

 

Edited July 1, 2021 by Flamboyant

[Flamboyant]

https://pubmed.ncbi.nlm.nih.gov/11769127/

Just now, Flamboyant said:

https://pubmed.ncbi.nlm.nih.gov/11769127/

[Immortalization of human fibroblasts using tsA mutant of SV40 and pSV3neo plasmid]

[Article in Russian]

L S Inozemtseva  1 , V G Chernikov, E S Manuilova, M I Marshak, N P Nikolaeva, O S Kulagina, I A Grivennikov

Affiliations

PMID: 11769127

Abstract

Clones of immortalized human fibroblasts with an extended life span in culture and a capability of subloning were obtained after the infection with a temperature sensitive mutant (tsA 239) of SV40 virus and pSV3neo plasmid. As compared with the parental cells, the obtained clones exhibited increased plating efficiency, decreased doubling time, and serum dependence. We did not obtained the colony formation during cultivation of immortalized cells in semiliquid agar. This means that our cells were not completely malignant. The PCR (polymerase chain reaction)-analysis has revealed the presence of viral DNA at early passages (25th passage) after the infection by tsA SV40, and its absence after a prolonged cultivation (46th passage). PCR-analysis of the clones obtained after pSV3neo transfection has revealed the presence of gene A sequences either at early (9-15), or later (62) passages. The expression of the gene A product in cells of these clones was revealed only early passages (11 and 35). Possible mechanisms of immortal phenotype origin in human diploid cells after the action of ts-mutant and other constructions of SV40 are discussed.

[Flamboyant]

What about https://en.wikipedia.org/wiki/Cryonics

Edited July 1, 2021 by Flamboyant

[Kartazion]

19 hours ago, Flamboyant said:

Always trolli,g ? A PhD trolling writing a book ?

Why publish in French on an English forum. It will not advance your point of view.
 

On 6/28/2021 at 5:34 PM, Flamboyant said:

Rhetoric question = useless.

A good question were : how about "longueur télomères" & "double DNA".

Good luck. Even excellent scientists cannot respond.

PS : photophobia =symptomatic.

More infos :

(I don't khow how to say it in english)

- Photophobia Allergy to sunlight 3rd degree
- Burns with SLE
- Vital need for blood transfusions - regardless of group.

Where comes from D antigen.

What is this gibberish?

 

[Flamboyant]

Sorry, I don't speak, "scientific" english very well.

Can a cool body explain me this ?

https://pubmed.ncbi.nlm.nih.gov/11769127/

It's Gov info.

And this : https://pubmed.ncbi.nlm.nih.gov/2153691/

PS : we have problems with light.

Edited July 1, 2021 by Flamboyant

[Kartazion]

2 hours ago, Flamboyant said:

Sorry, I don't speak, "scientific" english very well.

Can a cool body explain me this ?

https://pubmed.ncbi.nlm.nih.gov/11769127/

It's Gov info.

And this : https://pubmed.ncbi.nlm.nih.gov/2153691/

PS : we have problems with light.

Apparently, and as indicated, it concerns the imortality of certain cells in humans. Understanding this and the methods used requires several years of study.

[Flamboyant]

Thanks buddy. How to explain problems with light ?

As you can see :

 

https://pubmed.ncbi.nlm.nih.gov/15001238/

Immortalization of hepatic progenitor cells

Anne Weber  1

Affiliations

PMID: 15001238

DOI: 10.1016/j.patbio.2003.02.001

Abstract

Development of cell therapy-based strategies for the treatment of liver failures and of inherited metabolic diseases has become a necessity because of the limitations of orthotopic liver transplantation, including shortage of donor livers. This shortage limits also the availability for hepatocytes and these terminally differentiated cells cannot be expanded in vitro. Thus, other alternative sources of hepatocytes have to be explored such as hepatic stem cells. Foetal hepatic cells have specific intrinsic properties compared to adult hepatocytes that should overcome some of their limitations. Thus, the availability of in vitro expandable progenitor cells by means of immortalization and without inducing a transformed phenotype and disrupting their differentiation potential would facilitate studies on cell engraftment and differentiation within the hepatic parenchyma. A temporally controlled expression of the immortalizing transgene would also permit to revert the immortalized phenotype prior to cell transplantation. Since characteristics of murine stem cells cannot readily be extrapolated to their human or other primate counterparts, we have immortalized one clone of primate hepatic progenitor cells using a retroviral vector expressing SV40 Large T flanked by lox P sites. These hepatic cells were bipotent, expressing markers of both hepatocytic and biliary lineages. After transplantation into athymic mice, approximately 50% of immortalized cells engrafted, stopped proliferating after a few days and differentiated in adult hepatocytes, suggesting that the hepatic microenvironment plays an important role in such regulations. Upon infection with a retrovirus expressing the CRE recombinase, immortalized cells stopped growing and died, showing that immortalization was dependant on SV40 Large T. These studies suggest new approaches to expand hepatic progenitor cells, analyse their fate in animal models aiming at cell therapy of hepatic diseases.

[Flamboyant]

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.732.462&rep=rep1&type=pdf#page=17

WTF with light ?

And WTF with our liver ?

We're not so immortals.

Light can kill us.

Photophobia literally means "fear of light." If you have photophobia, you're not actually afraid of light, but you are very sensitive to it. The sun or bright indoor light can be uncomfortable, even painful.

Photophobia isn't a condition -- it's a symptom of another problem. Migraine headaches, dry eyes, and swelling inside your eye are commonly linked to light sensitivity.

It can cause pain whenever you're in bright sunlight or indoor light. You might want to blink or close your eyes. Some people also get headaches.

Causes

Photophobia is linked to the connection between cells in your eyes that detect light and a nerve that goes to your head.

Migraines are the most common cause of light sensitivity. Up to 80% of people who get them have photophobia along with their headaches. Many of those people are light sensitive even when they don't have a headache.

Other types of headaches can cause photophobia, too. People who get tension and cluster headaches also say they're uncomfortable around bright light.

A few brain conditions can cause photophobia, including:

• Meningitis (swelling of the protective coverings of your brain and spinal cord)
• Serious brain injury
• Supranuclear palsy (a brain disorder that causes problems with balance, walking, and eye movement)
• Tumors in your pituitary gland

Some eye diseases cause this symptom, including:

• Dry eye
• Uveitis (swelling of the inside of your eye)
• Keratitis (swelling of your cornea, the clear layer that covers the colored part of your eye)
• Iritis (swelling of the colored ring around your pupil)
• Cataracts (cloudy coverings over the lenses of your eyes)
• Corneal abrasion (a scratch on your cornea)
• Conjunctivitis (inflammation of the conjunctiva, the clear tissue that sits over the white part of your eye)
• Damage to your retina, the light-sensitive layer in the back of your eye
• Blepharospasm (a condition that makes your eyelids close uncontrollably)

Photophobia may also affect some people who have these mental health conditions:

• Agoraphobia (a fear of being in public places)
• Anxiety
• Bipolar disorder
• Depression
• Panic disorder

You can also get photophobia after you have LASIK or other surgery to fix vision problems.

Certain wavelengths of light -- like the blue light your computer and smartphone give off -- cause the most sensitivity.

Some medicines can also cause photophobia, including:

• Antibiotics, such as doxycycline and tetracycline.
• Furosemide (Lasix): This keeps your body from holding on to too much fluid. It's used to treat congestive heart failure, liver disease, kidney disease, and other conditions.
• Quinine (Qualaquin😞 This is a drug used to treat malaria.

Diagnosis

If you think you have photophobia, see your eye doctor. They'll ask about your symptoms and any medical conditions you have. Then they'll check the health of your eyes and possibly your brain.

Tests your doctor might use include:

• Slit-lamp eye exam. They'll use a special microscope with a light to examine your eyes.
• MRI, or magnetic resonance imaging.This uses powerful magnets and radio waves to make detailed pictures of your eyes.
• Exam of the tear film. This checks the amount of tears you make to see if you have dry eyes.

 

Treatment

The best way to ease photophobia is to treat the condition or stop taking the medicine that's causing it.

If you're still affected by it, tinted glasses may help. Some people have found relief from rose-colored lenses called FL-41.

But tinted lenses aren't for everyone. They can make some people even more sensitive to light, so talk with your doctor about what's best for you.

Edited July 1, 2021 by Flamboyant

[Flamboyant]

I see everyone is meaningfull.

As me.

As PhD's.

Edited July 2, 2021 by Flamboyant

[Kartazion]

6 hours ago, Flamboyant said:

I see everyone is meaningfull.

I see meaningless posts.

You talk about of development of cell therapy-based strategies for the treatment of liver failures and of inherited metabolic diseases has become a necessity because of the limitations of orthotopic liver transplantation, including shortage of donor livers, and ask about the light after posting a resident evil link.

23 hours ago, Flamboyant said:

WTF with light ?

???

Ask a clear and relevant question from your feed, ie the OP.
 

23 hours ago, Flamboyant said:

And WTF with our liver ?

When liver cells are attacked for a long time, they die and are replaced by fibrous tissue: this is “fibrosis”. "Cirrhosis" is the terminal phase of fibrosis.
 

23 hours ago, Flamboyant said:

We're not so immortals.

Light can kill us.

Photophobia literally means "fear of light." If you have photophobia, you're not actually afraid of light, but you are very sensitive to it. The sun or bright indoor light can be uncomfortable, even painful.

Photophobia isn't a condition -- it's a symptom of another problem. Migraine headaches, dry eyes, and swelling inside your eye are commonly linked to light sensitivity.

It can cause pain whenever you're in bright sunlight or indoor light. You might want to blink or close your eyes. Some people also get headaches.

Causes

Photophobia is linked to the connection between cells in your eyes that detect light and a nerve that goes to your head.

Migraines are the most common cause of light sensitivity. Up to 80% of people who get them have photophobia along with their headaches. Many of those people are light sensitive even when they don't have a headache.

Other types of headaches can cause photophobia, too. People who get tension and cluster headaches also say they're uncomfortable around bright light.

A few brain conditions can cause photophobia, including:

• Meningitis (swelling of the protective coverings of your brain and spinal cord)
• Serious brain injury
• Supranuclear palsy (a brain disorder that causes problems with balance, walking, and eye movement)
• Tumors in your pituitary gland

Some eye diseases cause this symptom, including:

• Dry eye
• Uveitis (swelling of the inside of your eye)
• Keratitis (swelling of your cornea, the clear layer that covers the colored part of your eye)
• Iritis (swelling of the colored ring around your pupil)
• Cataracts (cloudy coverings over the lenses of your eyes)
• Corneal abrasion (a scratch on your cornea)
• Conjunctivitis (inflammation of the conjunctiva, the clear tissue that sits over the white part of your eye)
• Damage to your retina, the light-sensitive layer in the back of your eye
• Blepharospasm (a condition that makes your eyelids close uncontrollably)

Photophobia may also affect some people who have these mental health conditions:

• Agoraphobia (a fear of being in public places)
• Anxiety
• Bipolar disorder
• Depression
• Panic disorder

You can also get photophobia after you have LASIK or other surgery to fix vision problems.

Certain wavelengths of light -- like the blue light your computer and smartphone give off -- cause the most sensitivity.

Some medicines can also cause photophobia, including:

• Antibiotics, such as doxycycline and tetracycline.
• Furosemide (Lasix): This keeps your body from holding on to too much fluid. It's used to treat congestive heart failure, liver disease, kidney disease, and other conditions.
• Quinine (Qualaquin😞 This is a drug used to treat malaria.

Diagnosis

If you think you have photophobia, see your eye doctor. They'll ask about your symptoms and any medical conditions you have. Then they'll check the health of your eyes and possibly your brain.

Tests your doctor might use include:

• Slit-lamp eye exam. They'll use a special microscope with a light to examine your eyes.
• MRI, or magnetic resonance imaging.This uses powerful magnets and radio waves to make detailed pictures of your eyes.
• Exam of the tear film. This checks the amount of tears you make to see if you have dry eyes.

 

Treatment

The best way to ease photophobia is to treat the condition or stop taking the medicine that's causing it.

If you're still affected by it, tinted glasses may help. Some people have found relief from rose-colored lenses called FL-41.

But tinted lenses aren't for everyone. They can make some people even more sensitive to light, so talk with your doctor about what's best for you.

What is the relationship between photophobia and the liver?

Make a technical effort with your posts and in relation to your questions.

[Flamboyant]

Surprise

[Phi for All]

!

Moderator Note

Two pages in, nobody knows exactly what you want to talk about. I'm throwing this thread in the Trash.

You can open another on the same topic, but our rules require that they make sense. Focus on what you want to ask, or discuss, or learn. Stop being vague. Stop commenting on things that aren't about the topic. Please stop making references to movies and games as it limits the circle of those who might be interested in discussing this with you. Posting links to videos with nothing to give them context is unhelpful, and against the rules.

I think you could use some help, but we're having trouble understanding you. The questions we can understand you asking require a LOT of technical language and medical knowledge, and it's difficult to know how much of that you understand (because of science knowledge, AND because of the obvious language barrier).

If you're just wanting to know more about a medical condition, start the thread in Anatomy, Physiology, and Neuroscience. If you insist you are immortal and want the chance to show supportive evidence, then post in Speculations. But please be more clear. Please.