stubbsey12 Posted July 29, 2022 Posted July 29, 2022 Is anyone able to explain this biphasic amplification that I've been seeing in my positive controls (Green curve)? For reference, this is using TaqMan probe technology, whose targets are simultaneously nsp16 and orf1ab (COVID-19). Please ask for further explanation! I cannot find any resources out there which can explain this..
CharonY Posted July 29, 2022 Posted July 29, 2022 It looks like unspecific amplification, especially considering how late in the cycle it comes up.
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