SevenCircles Posted October 29, 2022 Posted October 29, 2022 Hi, I'm new to Primer-BLAST. Can anyone help me, is this primer pair ok? Are the low GC% and high self complementarity of the minus strand problematic? What about the plus strand with 5.0 self complementarity and 4.0 self 3' self complementarity? Are the Tm too low? Thank you!
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