PCR of full Human DNA strand (extractet from mouth cells)

[Mycophone]

Dear ladys and gentleman,

I am very new to genetics, but regardless I got myself into the responsibility of designing a PCR workshop.

Now I struggle to find out what PCR-Primers I need if I want to multiply an entire DNA strand, rather than one specific sequence.

Can anyone help me please?

[CharonY]

If you are talking about a whole human chromosome, you can forget about it. Longest PCR products in one go using long range polymerase will get you maybe up to 40ish kb.

What is wrong with amplifying a smaller (more realistic) target?

Or are you thinking in terms of whole genome amplification? This is not standard pcr method and does not yield a singular product. It is more of a preamplification method to give more substrate for subsequent analyses.

[Lindsey123]

Mitochondrial DNA is relatively small (about 16.5kb depending on the species) and with some effort this can be amplified in one PCR reaction.