hilaryhurley Posted September 14, 2005 Posted September 14, 2005 can someone out there please donate a protocol for isolating human neutrophils from human blood. I did it about 8 years ago but cannot recall technique. involves dextran and ficoll but cannot obtain any detailed experimental protocol. stressed out.
zyncod Posted September 16, 2005 Posted September 16, 2005 I did it about a year ago. I think it was ficoll/hypaque. Fairly simple, but MACs would probably work better.
LucidDreamer Posted September 16, 2005 Posted September 16, 2005 " J. E. Coligan et al., Eds., Current Protocols in Immunology (Wiley, 1999)." If you have access to a library or online library.
sasu Posted October 13, 2005 Posted October 13, 2005 can anybody tell me process for breaking the neutrophils:mad:
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