hsiang Posted October 26, 2023 Posted October 26, 2023 I have a series of combination plasmids based on the pET-28a vector, each of which contains the ppk gene from different bacteria inserted at the same site. The sequence length of these plasmids is approximately 7300 base pairs each. I am curious about whether the copy numbers of these plasmids remain consistent after they are separately introduced into E. coli cells?
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