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Need help with neutrophil isolation from an inflammed mouse brain for FACS

smriti

By smriti
in Microbiology and Immunology

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smriti

smriti

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I need to extract neutrophils from an inflammed mouse brain for a FACS analysis, does anyone have a good protocol to do this? I am guessing I would have to use Percoll or Ficoll or something?

 

Thanks, best wishes. :cool:

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