rbc lysis

[sasu]

can anybody tell me goood method for rbc lysis:embarass:

[rakuenso]

what are you trying to extract? obviously not DNA.

[zyncod]

We use ammonium chloride with Tris base as the buffer. It works just as well as the commercial sol'ns and is a thousand times cheaper.

 

p.s. - Rakuenso, RBC lysis is used to remove red blood cells from cell suspensions as they interfere with flow cytometric analysis and tissue culture.

[sasu]

what are you trying to extract? obviously not DNA.

 

i want to separate rbc & wbc by chromatography

[sasu]

what are you trying to extract? obviously not DNA.

 

i want to separate rbc & wbc by chromatography

[rakuenso]

ohh i see

 

I thought you wanted to lyse individual RBCs and analyze its plasma contents

[sasu]

one simple que . we are using 5% dextrose & 0.9% NaCl solution as isotonic solution. but osmotic pressure of 5% dextrose 6.7 atm & 0.9% NaCl 3.7 atm. then why rbc get breaked if we use 3.7 atm of dextrose solution?

[Emily]

search for ACK lysis buffer.

[NPK]

Wouldn't plain water do the trick?

[zyncod]

No, plain water would lyse all the cells. You need a certain osmotic pressure to lyse the RBCs preferentially.