Helix Posted October 20, 2005 Posted October 20, 2005 I am about to start an experiment to keep me busy this weekend and I need a way to kill of yeast. Obviously there are many extreme ways to do this, bleach, nuclear warhead, hot flame among others, but I would like to use something more specific to organisms such as antibiotics. Would antibiotics be effective against yeast even though yeast is non-pathogenic? I would imagine it would have some effect. In any case, thanks for the help. I hope this project can stave off the Bordom demons and give me some insight into how microbes function.
ecoli Posted October 20, 2005 Posted October 20, 2005 there are certain chemicals that do a good job killing yeast... is that what you had in mind?
RyanJ Posted October 20, 2005 Posted October 20, 2005 Heat kills yeast by denaturing its enzymes thus renderind it useless. Also a high or low Ph would do the same thing If you could engineer an anti-body to attack and kill the yeast cells that would certainly stop them from working but thats probably way beond anything you could do for an experiment. Cheers, Ryan Jones
Mokele Posted October 20, 2005 Posted October 20, 2005 Monistat 7 and various other commerical yeast-infection treatements should do the trick.
Helix Posted October 21, 2005 Author Posted October 21, 2005 If you could engineer an anti-body to attack and kill the yeast cells that would certainly stop them from working but thats probably way beond anything you could do for an experiment. Wouldn't I love that? But that's way beyond the scale of a weekend project. That'd be interesting to do as a long-term project but I'm already entering a science fair so that might overload me. Monistat 7 and various other commerical yeast-infection treatements should do the trick. Thanks, I was wondering if yeast-infection medicines would work but I was worrried the "yeast" was slightly different. I'll go pick up some Monistat or similar item, which will be quite a buy considering I'm a guy.
Helix Posted October 21, 2005 Author Posted October 21, 2005 there are certain chemicals that do a good job killing yeast... is that what you had in mind? Sorry I didn't reply earlier to this, I didn't see it. Yes, anything like that. Actually chemicals would be good, if there is a chance that it wouldn't obliterate them, i.e. not kill them so well its not funny; give the yeast a fighting chance. In any case, does anyone know any other potential methods in case I want to try a few ways, besides monitstat and the chemicals? The other question remains, too: would any antibiotic or antibacterial work?
akcapr Posted October 21, 2005 Posted October 21, 2005 Arent yeast infections not actually due to yeast....
zyncod Posted October 21, 2005 Posted October 21, 2005 In your scenario, it sounds like you want to kill yeast without killing other organisms. Or you want to kill yeast in a particularly interesting way. I can recommend both; I just don't know what you want.
YT2095 Posted October 21, 2005 Posted October 21, 2005 pure Alcohol should kill yeast quite effectively, when yeast is used in brewing wine, it dies naturaly when the alcohol level gets to a certain concentration. so using pure alc will certainly kill even the hardiest of yeasts.
Helix Posted October 21, 2005 Author Posted October 21, 2005 Thanks, alcohol sounds good but it seems too good, for my project I need to give the yeast a chance to fight whatever I'm using, that's why I wanted to use antibacterials.
Lance Posted October 21, 2005 Posted October 21, 2005 It might be interesting to experiment with osmosis using a salt solution and pure water and then view the result under a microscope.
Helix Posted October 22, 2005 Author Posted October 22, 2005 That's an interesting follow up, how would I do that? Just add the salt water and pure water and view the osmosis?
Lance Posted October 22, 2005 Posted October 22, 2005 That's the way I would go about it, add either or. The salt should cause them to shrivel and the water should cause them to bulge. Here's an interesting page on osmosis: http://arbl.cvmbs.colostate.edu/hbooks/cmb/cells/pmemb/osmosis.html
zyncod Posted October 22, 2005 Posted October 22, 2005 Ok, so you specifically want to kill yeast. You can use the eukaryotic equivalents of antibiotics, things like cycloheximide and colchicine. Of course, the resistance rate is going to be much lower than bacterial antibiotic resistance rates since eukaryotes are far more complex.
BioNerd Posted October 30, 2005 Posted October 30, 2005 Yeast is a fungus. How would antibiotics do anything?
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