isolation of a target gene from a genome

[satokaiba]

Dear all,

I sm going to isolate the NOX gene from S.mutans, however i am still not sure about the procedure. Could u please give me a protpcol for that. Thanks a lot.

One more question that: what is gateway system and please explain more for me

Yours scincerely,

satokaiba

[ecoli]

first thing to do is to design primers specific to your gene. Know how to do that?

 

You'll have to buy software for this if its not a common gene.

[satokaiba]

thank you so much for your time,

I did design the primers for that gene and used NcoI and PstI as the restriction site for it. However, i still wanna have a procedure to do experiments in the lab. Please help:-)

NOX gene is NADH oxidase gene and S.mutans is Streptococcus mutans.

 

satokaiba