Yggdrasil Posted February 5, 2006 Posted February 5, 2006 In biochemistry, we use devices which use the principle of surface plasmon resonance to study the binding between biological molecules. Even though I uses these machines, I'm still not to clear on how exactly they work. All I know that shining monochromatic, plane polarized light on a thin metal boundary between two materials with different indices of refraction generates a "evanescent wave" which will reduce the intensity of the totally internally reflected light at a specific angle, but I'm not exactly sure what the "evanescent wave" people talk about is nor why it reduces the intensity of the reflected light at a specific angle. Any clarification on these issues would be appreciated.
Klaynos Posted February 5, 2006 Posted February 5, 2006 If it works in the way I am guessing just from reading your description, you want to read up on "bragg reflections" and crystal lattices...
Yggdrasil Posted February 7, 2006 Author Posted February 7, 2006 If by Bragg relflections, you mean Bragg diffraction, I don't think that's relevant since SPR isn't used to calculate distances. SPR is used to measure the index of refraction of a layer of biological molecules, which is proportional to the mass of the molecules in that layer.
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