Kinase Activation Assays?

[bayoubelle]

I am trying to find a way to detect whether a specific kinase is activated or not. Any good kits or procedures for how to do this?

[qed]

I am trying to find a way to detect whether a specific kinase is activated or not. Any good kits or procedures for how to do this?

 

if you have purified substrate protein/peptide, you can measure the activity via standard enzym kinetics. Other possibility is to use radioactiv P as a tracer and detect the phosphorylation of your substrate.

[Yggdrasil]

Is this an in vitro or in vivo experiment?