Dak Posted February 28, 2006 Posted February 28, 2006 Is anyone aware of a restriction enzyme database that is easyer to search than rebase? eg, i want to find a restriction enzyme that... has a recognition sequence that:doesnt start or end in C doesnt contain a CG or GC is palendromic [*]works at 37oC [*]has been sequenced [*]has a methylase that has been sequenced [*]has a methylase that methylates cytosine [*]and prefferably... has a methylase that metylates cytosine somewhere other than carbon-5 is small (both restriction enzyme and methylase) and cuts to leave blunt-ends Now, i dont mind a certain level of reading through lists till i find what i want, but so far, i cant think of anyway to find what i want other than just reading through the list of every enzyme in rebase till i find what i want. which would suck. so, is anyone aware of a restriction-enzyme database that has a better search-feature than rebase?
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