Enzyme Test

[leidiot]

My friends and I are doing a project on digestions and it would help if we could find out how to test for enzymes. Thanks:-)

[chuinhen]

Enzymes are specific that is they react with certain pH , certain substrate .

 

Thus we can manipulate the above variables to test the enzyme .

 

FOR EXAMPLE ( CONSTANT TEMPERATURE , pH)

 

we have enzyme a , b , c to test .

 

1)we add 5cm egg slice into ezyme a , b , c in different beakers with equal volume .

 

2)After 30 mins . measure the length of each egg slices .

 

Protease digest the protein content in the egg ,

thus the beaker which has shorter egg slices contains Protease .

[RyanJ]

Actually I would use carbohydrase and bread - same method except you weigh the bread (making shure its dry so the water does not affect the results).

 

length is a problem because the enzymes will catalyse any surface so hight could decrease instead of length which is why weight is probably better

 

Cheers,

 

Ryan Jones