DNA Break Up

[KFC]

I've isolated some DNA in ethanol(70% Solution) from Split Pees is there a way to separate ß-2-Deoxy-D-ribose, Thymine, Adenine, Guanine, Cytosine, TMP, AMP, GMP, or CMP.

[woelen]

How are you sure you isolated DNA? Don't you have any other organic crap from the cells, other organic matter, etc. ? It seems quite difficult to me to isolate DNA.

[KFC]

http://learn.genetics.utah.edu/units/activities/extraction/

 

http://biology.about.com/c/ht/00/07/How_Extract_DNA_Human0962932481.htm

 

Not sure if its pure it probably has some impurities like RNA or Enzymes or something.

[insane_alien]

you probably have a lot of impurities.

[woelen]

Using such a simple procedure definitely gives you very impure DNA. It will be hard to separate the DNA from the other organic stuff.

[Karnage]

why don't you use lab equipment to isolate DNA? This method you use is quite simple and doesn't yield a high percentage of pure DNA

[insane_alien]

maybe because he doesn't have lab equipment?

[John Cuthber]

I think that procedure probably gives reasonably clean nucleic acid- certainly if there were a lot of trash in it, it wouldn't be a stringy goo like DNA.

I think it's not too dificult to hydrlyse DNA to its constituents. Enzymatic hydrolysis would be neatest but I think a strong base would do.