sheanhung Posted November 6, 2006 Share Posted November 6, 2006 Materials: 6M HCl and protein solution Condition : nitrogen gas atmosphere (to prevent oxidation), 150 degree celcius for 65 mins. What apparatus or instruments to be used to do this hydrolysis? Link to comment Share on other sites More sharing options...
ecoli Posted November 6, 2006 Share Posted November 6, 2006 western blots are for proteins, but if your breaking them down, I don't know how that would work. Amino acid chromotography maybe? Link to comment Share on other sites More sharing options...
Bluenoise Posted November 6, 2006 Share Posted November 6, 2006 Yeah with those conditions you'll just end up with amino acids. Well you'll definatley need some acid resistant pressurized container. Link to comment Share on other sites More sharing options...
sheanhung Posted November 8, 2006 Author Share Posted November 8, 2006 If I perform classical acid hydrolysis using same materials, the hydrolysis temperature would be 110 degree celcius and time frame is 24 hours and above. Can I perform the classical acid hydrolysis in separate days (3 days, 8 hours per day)? Link to comment Share on other sites More sharing options...
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