Acid hydrolysis of protein

[sheanhung]

Materials: 6M HCl and protein solution

 

Condition : nitrogen gas atmosphere (to prevent oxidation), 150 degree celcius for 65 mins.

 

What apparatus or instruments to be used to do this hydrolysis?

[ecoli]

western blots are for proteins, but if your breaking them down, I don't know how that would work. Amino acid chromotography maybe?

[Bluenoise]

Yeah with those conditions you'll just end up with amino acids.

 

Well you'll definatley need some acid resistant pressurized container.

[sheanhung]

If I perform classical acid hydrolysis using same materials, the hydrolysis temperature would be 110 degree celcius and time frame is 24 hours and above.

 

Can I perform the classical acid hydrolysis in separate days (3 days, 8 hours per day)?