Enzyme purification: fill in the blanks

[D_C_]

Fill in the blanks.

 

The first workable fraction in the enzyme purification is the __________. Each subsequent successful purification step should show an increase in ____________ and very little decrease in __________________. To keep track of all proteins during each step, we would typically run a general protein assay, such as the ________, which utilizes the dye Coomassie Blue, which binds to ___________ groups in proteins.

 

What I have so far:

 

1. Crude

2. Not sure if it's specific activity or fold purification.

3.

4. Bradford assay

5. Hydrophobic

[shel]

2 should definitely be specific activity. I would guess that 3 is total solute concentration but I'm not so sure

[Bluenoise]

I'd say 3 is target concentration for lack of a better word.

[ho&ho]

2.specific activity or purifiction (i think both r right)

3. yield