immunologists

[christine]

hi i have a question for any immunologists,

does any one know of any protein, detectable by western blot, that are localised to the T cell nucleus regardless of T cell activation?

I'm doing a cell fractionation and i need to confirm that the nuclear fraction does indeed contain the nucleus. thanks.

[Bluenoise]

In humans?

 

Do you mean on the exteriour of the nucleus? I'd imagine some part of the nuclear pore complex would be a safe bet. And all cells with a nuclues must have this complex for the nuclues to function so it should work for T-cells.

 

*Edit*

 

Good guess on my part. I found a company selling antibodies for detection of the nucleus by porins in the nuclear pore complex in humans.

 

http://www.novusbio.com/data_sheet/index/&prod_id=20470&prev_module=search

 

Found another one here as well, uhhh this one is for yeast, so it might not work for humans. Says not tested in humans.

 

http://www.google.ca/url?sa=t&ct=res&cd=7&url=http%3A%2F%2Fwww.abcam.com%2Findex.html%3Fdatasheet%3D4641&ei=v12kRab0B6DQgQKdsr2eBA&usg=__09rofEzS9rqMStl4HqaeXWpztBk=&sig2=a-PsVJXa81hCZWCSpQQcCw

 

Hope that helps.

[Bluenoise]

Hmmmm no response or any comments from anyone else??

[queenc]

Here's are some sources that I found. 

http://www.genetex.com/LC3B-Protein-GTX116080-pro.html

http://www.genetex.com/LC3-Protein-GTX118992-pro.html

These are for western blot with reactivity in human. I would confirm with their technical support if they are localised to the T cell nucleus regardless of T cell activation.

 

Best of luck!