Lola Vargas Posted February 16, 2007 Posted February 16, 2007 Hi, I have a question regarding antibody concentration in immunized rats. When immunizing them with a recombinant protein, what's the "expected" concentration of IgG to my protein? I understand this must differ a lot (!), but does anyone have a feeling for what a reasonable concentration range might be? Are we talking mg/ml, ug/ml, ng/ml? What's the avarage concentration of total IgG in a rat? 3-20mg/ml? Cheers,
Lola Vargas Posted March 4, 2007 Author Posted March 4, 2007 Doesn't ANYONE know this?!? Very surprising.... Totally useless...
badchad Posted March 4, 2007 Posted March 4, 2007 If you're using a recombinant protein, the amount of IgG formed will be relative to the immune response generated against the protein. Theoretically, a benign protein wouldn't generate much of a response and you could inject tons of protein into your rat and get nothing. As you stated, there is absolutely no way to tell. I suggest looking at some previously published literature.
JackMuChabas Posted August 17, 2007 Posted August 17, 2007 Yes there is a way to tell by using the gluco protein response and surmising it level of deterrence.
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