question on apoptsis

[immortal]

Hi,

can anyone tell me how nitric oxide[NO] causes programed cell death by blocking the FAS pathway.

[Hoose]

Can you be a little more specific because it really depends on which protein you are talking about (ie. nNos, eNos, iNos) that forms NO resulting in oxidative toxicity. I also don't think that FAS ligand and FAS intteraction is actually blocked by NO since both are trying to initiate programmed cell death (apoptosis). NO is harmful for the cell and its production results in intracellular degradation and FAS pathway recruits in death domains that initiate a nuclease that is able to enter the nucleus and cleave DNA.

[immortal]

I am talking about the enzyme casparase.

[immortal]

I apologise it is caspase not casparase.

[Hoose]

Well, I'm pretty sure FAS ligand downstream components a cleave procaspase 3 into caspase 3 which cleave ICAD into CAD, which enters the nucleus to chop up DNA, but I still don''t this NO blocks FAS pathway.