sybr green buffer

[mutation]

Hi everyone,

I am new in Q-PCR, and using My own enzymes and dNTP mix. I do have everything what are in Kit contents but not SYBR Green buffer. I need SYBR Green buffer's recipe to start my experiment.

I will be very glad if someone helps me with the recipe.

thanks

 

mutation

[CharonY]

The buffer does not depend so much on the dye but rather on the polymerase that you use. Most polymerase buffer consist of a basic Tris-buffer (ph 8-8.8), Mg, and a mix of KCl and (NH4)2SO4. Other ingredients (e.g. BSA, Triton X-100, betaine etc. are possible but not essential).

 

In short, if you use your own polymerase, just add the buffer for that enzyme.

[Bluenoise]

Ditto.

Just setup the reaction like any other PCR but with the addition of an appropriate amount of Sybrgreen.