The use of two carrier proteins for antibody production?

[Dynamite]

I have produced polyclonal antibodies in the past to synthetic peptides... I have had varying degrees of success but have a few peptides that consistently give low titres when conjugated to KLH.

 

It has been suggested that I perhaps use the peptides conjugated to two carrier proteins say KLH and then another.... the person who suggested it advised me to imunise using KLH then boost with the other peptide conjugated to the alternative carrier and then continue to alternate with each boost.

 

This is something he has apparently read and the paper claimed to increase titre levels, he has not performed this method himself! has anyone got any experience or thoughts on this method?

 

Thanks

 

D

[Prewitt]

i don't have much experience with antibody production but it seems quite reasonable titres increase. you have plenty of epitopes on the carrier protein. but since only the ones for your hapten-like (appearently) protein really matter i'm not sure it gives you better antibodies. but that's just speculation.