latkan Posted October 7, 2007 Share Posted October 7, 2007 I need help to figure out a method of obtaining a DNA fragment containing the full coding sequence for human rhodopsin. Also does anyone know how you would determine the number and size of rhodopsin transcripts in the human retina?? I have found out that the human retina has 3 rhodopsin transcripts sized 2.8,1.9 and 1.5kb. can someone tell me about the mechanism that produces these and what the functional significance might be??? Thx In advance Link to comment Share on other sites More sharing options...
ecoli Posted October 7, 2007 Share Posted October 7, 2007 design a primer and PCR a sample of human DNA. Link to comment Share on other sites More sharing options...
CharonY Posted October 8, 2007 Share Posted October 8, 2007 Well, which methods of mRNA quantification do you know? Also, what mechanisms do eukaryotes possess to generate different transcripts from the same gene? Link to comment Share on other sites More sharing options...
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