Uni Help: Need help on rhodopsin asap.

[latkan]

I need help to figure out a method of obtaining a DNA fragment containing the full coding sequence for human rhodopsin.

 

Also does anyone know how you would determine the number and size of rhodopsin transcripts in the human retina??

 

I have found out that the human retina has 3 rhodopsin transcripts sized 2.8,1.9 and 1.5kb. can someone tell me about the mechanism that produces these and what the functional significance might be???

 

Thx In advance

[ecoli]

design a primer and PCR a sample of human DNA.

[CharonY]

Well, which methods of mRNA quantification do you know?

Also, what mechanisms do eukaryotes possess to generate different transcripts from the same gene?