Dendritic cells-T cells, Tregs

[batban]

Hello,

 

I have a question regarding regulatory experiments using generated regulatory T cells and adding them to different DC:T (responder) cells allogeneic cultures. The problem is that by adding Tregs, you also dilute the existing DC:T(resp.)cell ratio and thereby automatically reducing cpm counts.

Does anyone have an idea how to cope with this, becasue in the literature they don't seem to have a problem with it, but I do. When they put control effector cells instead of Tregs, it doesnt lower their cpm count, although the number of effector T cells is as high or higher than that of responder T cells.

 

Thank you very much,

 

best regards.