Calculating Km and Vmax

[mel49]

Hi,

I have a practical to write up, have managed to plot a graph with lines to show Vmax plus 1/2 Vmax...the problem I have is the maths bit, I simply canot remember what I need to do.

So far I have worked out Km.....say is 0.75mM on my graph.....substrate concentration e.g is 2.4mM what calculations do I need to do so I can proceed to the next stage....as I will have to do a Linewaver Burk plot afterwards. Any help gratefully received.

 

Mel

[Testo]

You may have wanted to mention that this is an enzyme kinetics question. In a lineweaver/burk plot u plot 1/[substrate concentration] along the x-axis and 1/velocity on the y-axis, giving u a linear plot. The gradient of this is Km/Vmax and the y-intercept is 1/Vmax you now have everything you need to get both quantities.