trypsin on casein hydrolysis

[garytse86]

Hello there. I have just finished a biological experiment on "effect of trypsin concentration on rate of casein hydrolysis"

I have already obtained a graph, and I used a program called "Graphpad Prism" to analyse the data usin nonlinear regression (3rd degree polynomial). I have got all the parameters like sy.x, degrees of freedom... But how do I use these parameters to analyse whether my results are reliable, and by varying enzyme concentration results in an increase in rate?

 

I have also considered Spearman's rank coefficient but this is only for linear relationships. What else can I do to determine whether my results show regression?

[Skye]

I'm not really sure, maybe it's possible to transform the data and do a linear regression?

[garytse86]

but don't you lose a lot of the accuracy if you do the transformation?