Culturing PBMC with LPS stimulation

[Sly3000]

Hello all.

 

I was wondering how much (what concentration) of LPS per ml of cell-medium, do you have to use to keep the PBMC culture alive and proliferating.

 

The PBMC is from a healthy donor.

 

Thanks in advance.

[zule]

I think it would depend on the proliferation speed you want to get, but an appropriate amount could be 50 ng/ml.

[Sly3000]

I think it would depend on the proliferation speed you want to get, but an appropriate amount could be 50 ng/ml.

 

Thanks for your reply.

 

Well, if you add too much to the culture the cells can also die. So that's what I want avvoid. I've added 1 microg/ml and that's a whole lot more than you suggest!!!