can anyone help me figure these out? I included all formulas.

[stew]

Note: a Unit is ((ΔA/Δmin)/(6220 M -1 cm -1)) X 106 μM/M X 3 X 10-3 L

 

An enzyme sample contains 24 mg protein/mL. Of this sample, 20

microliters in a standard incubation volume of 0.1 mL catalyzed the

incorporation of glucose into glycogen at a rate of 1.6 nmol/min. Calculate the

velocity of the reaction in terms of the following:

 

A)micromoles/minute

B)micromoles/liter/minute

C)micromoles/miligrams of protein/minute

D)Units/milliliter

E)Units/miligrams of protein

 

 

Of this sample, 50 mL were fractionated by ammonium sulfate

precipitation. The fraction precipitating between 30 and 50% saturation was

redissolved in a total volume of 10 mL and dialyzed. The solution after

dialysis had 12 mL and contained 30 mg protein/mL. Of the purified fraction,

20 microliters catalyzed the reaction rate of 5.9 nmol/min under the

standard assay conditions. calculate the following:

 

A. The recovery of enzyme after the ammonium sulfate step.

B. The fold purification after the ammonium sulfate step.

 

note: for recovery: divide total units of he sample by the total units of the crude homogenate and multiply by 100.

for fold purification: it is the ratio of the specific activity of a given fraction divided by the specific activity of the crude homogenate.

 

a Unit= micromole/minute