MALDI-TOFTOF Posted April 5, 2008 Share Posted April 5, 2008 How come IgG isotypes are used as secondary antibody for detection when performing a serum-ELISA and not IgM? It says so in a course immunology, but I can not find the underlying reason. Link to comment Share on other sites More sharing options...
CharonY Posted April 8, 2008 Share Posted April 8, 2008 You generally use polyclonal antibodies as second ones. These happen to be IgGs Link to comment Share on other sites More sharing options...
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