MALDI-TOFTOF Posted April 5, 2008 Posted April 5, 2008 How come IgG isotypes are used as secondary antibody for detection when performing a serum-ELISA and not IgM? It says so in a course immunology, but I can not find the underlying reason.
CharonY Posted April 8, 2008 Posted April 8, 2008 You generally use polyclonal antibodies as second ones. These happen to be IgGs
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