YT2095 Posted April 7, 2004 Share Posted April 7, 2004 you`ll certainly need to give it plenty of time, also take into account that "Lime jelly" has preservatives in it that inhibit this type of activity I`de give it at least a week as a fair trial, by then you`ll see something if your going to Link to comment Share on other sites More sharing options...
aommaster Posted April 7, 2004 Share Posted April 7, 2004 what should he actually see when the bacteria grow properly? I did an experiment simimlar to this one using agar, and i saw white 'spots' of colonies of bacteria. IS that what he is supposed to see? Link to comment Share on other sites More sharing options...
YT2095 Posted April 7, 2004 Share Posted April 7, 2004 yup white green blue black grey some are even red! it`ll apear as an inconsistency to the surface of the gel, and then gradualy become more profound over time. the neat thing with the fingerprint sample, you`ll actualy see the print after a while Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 7, 2004 Author Share Posted April 7, 2004 Cool:) thanks for the advice Link to comment Share on other sites More sharing options...
YT2095 Posted April 7, 2004 Share Posted April 7, 2004 not a prob I accept Visa, Master Card, or Reputation points -1 Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 8, 2004 Author Share Posted April 8, 2004 Ummmmm, I am not sure if i see signs of bacteria, I see a VERY VERY faint (could be anything) cloud. I have added these nutrients to my gelatin: Sugar adipic acid sodium citrate fumaric acid a tiny bit of salt Could any of those be stopping the bacterium from growing????????? Are they way too acidic???? Help will be appreciated Link to comment Share on other sites More sharing options...
aommaster Posted April 8, 2004 Share Posted April 8, 2004 how long have u incubated it? Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 8, 2004 Author Share Posted April 8, 2004 it has been a day now, in the low 90 deg. F. EDIT: And it has been 1 and 1/2 days, I still cease to notice any growth?? Can YT explain this? I think there is only one culpret for this, and that is the culture medium. Did i put in bad nutrients, as stated above (I have added these nutrients to my gelatin: Sugar adipic acid sodium citrate fumaric acid a tiny bit of salt) Link to comment Share on other sites More sharing options...
Dave Posted April 8, 2004 Share Posted April 8, 2004 Probably needs longer to be honest with you. I'd say that there's probably a number of factors involved in bacteria reproduction, and although I'm not a biologist, I'd probably give it a bit more time. 1 Link to comment Share on other sites More sharing options...
aommaster Posted April 9, 2004 Share Posted April 9, 2004 for my bacteria sample, we left them for about a week before seeing tiny spots! Link to comment Share on other sites More sharing options...
Dave Posted April 9, 2004 Share Posted April 9, 2004 Well you don't know what bacteria you're actually cultivating, and I'd think that some bacteria is slower to cultivate than others anyway. Link to comment Share on other sites More sharing options...
aommaster Posted April 9, 2004 Share Posted April 9, 2004 must be. We got out bacteria from our hands! Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 9, 2004 Author Share Posted April 9, 2004 hmmm, thanks for the reassurence that it will take longer, but still come Link to comment Share on other sites More sharing options...
aommaster Posted April 9, 2004 Share Posted April 9, 2004 it should. The experiment that u specified looks fine. Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 10, 2004 Author Share Posted April 10, 2004 YAHOOOOOOOOOOOOOOO I see my first two dots! I think they are some colenies!!!! (Pictures comming soon) Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 10, 2004 Author Share Posted April 10, 2004 Ok, it turneed out tobe alittle more then 2 colonies, it seems i was not looking quite close enough Link to comment Share on other sites More sharing options...
YT2095 Posted April 10, 2004 Share Posted April 10, 2004 excellent! what ever you do though, do NOT take the lid off! and always wash your hands afterwards with dillute chlorox bleach. don`t breathe any of it in! I`de like to see the results of your experiments for many years to come, not have you laid up in hospital somewhere! Link to comment Share on other sites More sharing options...
aommaster Posted April 10, 2004 Share Posted April 10, 2004 excellent! what ever you do though' date=' do NOT take[/quote'] is that just to keep the bacteria inside the container? or is there another reason? Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 10, 2004 Author Share Posted April 10, 2004 Yes, I have come very prepared for that (as in safety). Are wearing rubber glooves, a docters mask (my dad is a docter, so i have plenty), goggles, and the frequent washing of hands enough? (w/ diluted bleach and anti-bacterial soap, bleach not to be mixed w/ ammonia ) PS, i think the otherr reason is SAFETY!!! Link to comment Share on other sites More sharing options...
YT2095 Posted April 10, 2004 Share Posted April 10, 2004 is that just to keep the bacteria inside the container? or is there another reason? yeah, you really don`t want spores to enter your lungs, or other airborne spores to contaminate your dish either, mayny often have a bit of sellotape around the lid too, in case it gets dropped (accidents happen). but basicly it keeps you and your sample safe Link to comment Share on other sites More sharing options...
Sayonara Posted April 10, 2004 Share Posted April 10, 2004 The air is filled with bacteria and spores - you also don't want those settling on your medium and changing your results. Link to comment Share on other sites More sharing options...
DeoxyriboNucleicAcid Posted April 10, 2004 Author Share Posted April 10, 2004 Very true Sayonara<sup>3</sup> Link to comment Share on other sites More sharing options...
Dave Posted April 10, 2004 Share Posted April 10, 2004 It's very important to keep the petri lid on; especially since you don't know what bacteria you're actually cultivating - some of that stuff could be quite nasty if inhaled. Link to comment Share on other sites More sharing options...
aommaster Posted April 10, 2004 Share Posted April 10, 2004 what effects could cultivating unknown bacteria cause? Serious illness? Damage to the body? Link to comment Share on other sites More sharing options...
YT2095 Posted April 10, 2004 Share Posted April 10, 2004 that`s just it, Unknown but most are not pleasant! Link to comment Share on other sites More sharing options...
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