need help with determining proteins in medium

[jaf572]

What is the most reliable/reasonable way to go about searching for unknown proteins in conditioned medium.

I have cultures of cells and need to look at soluble proteins released from these cells. I'm looking for relatively small proteins. I'm starting from scratch.

[CharonY]

What equipment do you have access to and do you just want to quantify them, or really identifying them? In the latter case you probably need an MS or a tandem/MS.