how to measure NR3C1 concentration in sputum?

[bekble]

Hi everyone!

 

I'd like to measure concentration of NR3C1 in sputum of asthmatics.

 

I suppose real time PCR s the best method and becase of that I have some questions.

 

What do I need to do this?

I found primesr for this receptor.

http://www.realtimeprimers.com/vhps-1818.html

 

what else do I need to start and where can I read more about how to do this.

 

Of course I have an access to lab but I'd like to read on my own before asking my assistant professor everything.

I am a beginer in molecular technics.

 

hope to read from you.

best wishes.

[Monomer]

what else do I need to start and where can I read more about how to do this.

 

The internet!!

 

Go a google search on RT-PCR, and check out google scholar and pubmed to find out what others have done. That's what I do when I'm starting on a new technique that I'm unfamiliar with.

 

Certainly take the initiative and gather as much information on your own, but if you're a beginner then don't feel bad about asking your professor questions.

Edited October 15, 2008 by Monomer
Added more info.