Botaras Posted December 10, 2008 Share Posted December 10, 2008 guys i have a question... why did you use acetone and not water for pigment isolation?? Link to comment Share on other sites More sharing options...
John Cuthber Posted December 10, 2008 Share Posted December 10, 2008 I didn't. Link to comment Share on other sites More sharing options...
Mokele Posted December 11, 2008 Share Posted December 11, 2008 Just a guess, but are you evaporating off the acetone at any point? If so, it may be used simply because the temperature needed to evaporate off water in a reasonable time would damage the pgiments. Link to comment Share on other sites More sharing options...
MedGen Posted December 20, 2008 Share Posted December 20, 2008 If you're trying to extract hydrophobic photosynthetic pigments then you need to employ a hydrophobic solvent. I'm guessing you'll be using chromatography, thin-layer perchance? Link to comment Share on other sites More sharing options...
Holyboy27 Posted December 22, 2008 Share Posted December 22, 2008 when i conducted the chromatography experiment, acetone is used to break down the cell wall... isn't it? Link to comment Share on other sites More sharing options...
Mokele Posted December 22, 2008 Share Posted December 22, 2008 Physical grinding can usually break up the cell walls perfectly well. Link to comment Share on other sites More sharing options...
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