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Extended Spectrum B lactamases


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Hi everyone

 

i am new to this forum. I was hoping to get opinion on how people test for these resistance mechanisms in the first line and then what further testing the carry out to confirm.

 

Also what experiences do people have of them

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Hi White Cat

 

The easiest way is to first check for cefpodoxime resistance, then use cefpodoxime/clavulanate disc diffusion (10 + 1ug discs) to check for synergy in all cef resistant isolates. If the cef/clav zone is 5mm or more larger than cef alone, the isolate is ESBL positive. This is the BSAC recommendation as well, which is the best thing to use if you are working in the UK

 

Hope this helps.

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