MicroGirl Posted January 23, 2009 Share Posted January 23, 2009 Hi everyone, I made ampicillin-LB media on Wednesday and poured it into plates on wednesday afternoon. After allowing them to set i turned the plates over and left them on my desk wednesday night, Thursday day and Thursday night:doh:. This morning (Friday) i have packed them and put them in the fridge. I was just wondering, was it a mistake to have left them a whole day at room temperature? Has my media or the ampicillin gone off over this time? Can i still use my plates or should i just make new ones? Thanks heaps for your help, Christy. Link to comment Share on other sites More sharing options...
CharonY Posted January 23, 2009 Share Posted January 23, 2009 One day only? Should not do anything. Remember, you are likely to incubate your bacteria for at least one day (if it is E. coli) at 37° anyway. Most ABs are fairly stable. While the efficiency might go down, you usually have so much of it in that it does not matter. It is a different thing if you work near the minimal effective concentration, though. In that case you have to keep everything as similar as possible. Link to comment Share on other sites More sharing options...
MicroGirl Posted January 30, 2009 Author Share Posted January 30, 2009 Thanks very much! Its probably alright then Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now