Has My media gone off?

[MicroGirl]

Hi everyone,

 

I made ampicillin-LB media on Wednesday and poured it into plates on wednesday afternoon. After allowing them to set i turned the plates over and left them on my desk wednesday night, Thursday day and Thursday night:doh:. This morning (Friday) i have packed them and put them in the fridge.

 

I was just wondering, was it a mistake to have left them a whole day at room temperature? Has my media or the ampicillin gone off over this time? Can i still use my plates or should i just make new ones?

 

Thanks heaps for your help,

Christy.

[CharonY]

One day only? Should not do anything. Remember, you are likely to incubate your bacteria for at least one day (if it is E. coli) at 37° anyway. Most ABs are fairly stable. While the efficiency might go down, you usually have so much of it in that it does not matter. It is a different thing if you work near the minimal effective concentration, though. In that case you have to keep everything as similar as possible.

[MicroGirl]

Thanks very much! Its probably alright then