c.lavancio Posted March 16, 2009 Posted March 16, 2009 Would anyone know how to stain proteins prior to gel-loading? I am looking for a homemade dye, i.e. Involving reagents that can be found in the lab and not by ordering from a company. Thanks.
CharonY Posted March 16, 2009 Posted March 16, 2009 I guess you are looking for a coomassie type prestain? I do not use them myself, but you essentially you can use coomassie under acidic conditions to your sample. Check out Judd 1979 Analytical Biochemistry as an example.
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